FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6947
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG140.20152583849143516No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC110.15834173024327047No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG110.15834173024327047No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA110.15834173024327047No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA100.14394702749388225No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT100.14394702749388225No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT90.12955232474449402No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC90.12955232474449402No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC90.12955232474449402No Hit
GTATGGAGACATATCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTT80.1151576219951058No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA80.1151576219951058No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA80.1151576219951058No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG80.1151576219951058No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT80.1151576219951058No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80.1151576219951058No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT70.10076291924571758No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA70.10076291924571758No Hit
CTTTAGTAGGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA70.10076291924571758No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC70.10076291924571758No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC70.10076291924571758No Hit
GGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTA70.10076291924571758No Hit
GCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTA70.10076291924571758No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT70.10076291924571758No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC70.10076291924571758No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT70.10076291924571758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)