FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6340
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAG110.17350157728706625No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG100.15772870662460567No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG100.15772870662460567No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC80.12618296529968456No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT80.12618296529968456No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG80.12618296529968456No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC70.11041009463722397No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC70.11041009463722397No Hit
CTTTAGTAGGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA70.11041009463722397No Hit
ATATTGTACGGTACCATAAATACTTGACCACCTGTAGTACATAAAAACCC70.11041009463722397No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT70.11041009463722397No Hit
CTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTA70.11041009463722397No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT70.11041009463722397No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG70.11041009463722397No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC70.11041009463722397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGCC100.008399709135.936518
TTATAGA100.008399709135.936514
TATAGAT100.008399709135.936515
GTTGCCT100.008399709135.936519
GGGGGGG200.00820466227.18730225-29
Produced by FastQC (version 0.11.5)