FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76393
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1060.1387561687589177No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC980.1282840050789994No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT980.1282840050789994No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA940.12304792323904024No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT920.12042988231906065No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG890.11650282093909128No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC850.11126673909913212No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT840.10995771863914233No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA810.10603065725917296No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA790.1034126163391934No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC780.1021035958792036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)