FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15243
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1080.7085219444991143No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG320.2099324279997376No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT250.16400970937479498No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT200.131207767499836No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA190.1246473791248442No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC160.1049662139998688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGAT250.009230292131.50476145
GTAATAG300.004810436657.3421941
TTTTTTT650.0024829434.597843140-144
CCCCCCC1550.001933398410.480455125-129
Produced by FastQC (version 0.11.5)