FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32430
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5031.5510329941412273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATTT350.0043354128169.19826145
TGATTAG400.0064635836148.04848145
GATAATA450.009191747131.59865145
Produced by FastQC (version 0.11.5)