FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13468
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC340.25245025245025243No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG300.22275022275022277No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC290.2153252153252153No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA260.19305019305019305No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT230.17077517077517076No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA220.16335016335016334No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA210.15592515592515593No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC210.15592515592515593No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC210.15592515592515593No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT200.1485001485001485No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC200.1485001485001485No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA200.1485001485001485No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG200.1485001485001485No Hit
CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCA180.13365013365013365No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT180.13365013365013365No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC180.13365013365013365No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG180.13365013365013365No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT170.12622512622512622No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACA170.12622512622512622No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC170.12622512622512622No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT160.11880011880011879No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT160.11880011880011879No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA150.11137511137511139No Hit
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG150.11137511137511139No Hit
CAACAGTACATAGCACATATAGCCATTTACCGTACATAGCACATTACAGT150.11137511137511139No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC150.11137511137511139No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG150.11137511137511139No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT150.11137511137511139No Hit
CTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAA150.11137511137511139No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT150.11137511137511139No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT140.10395010395010396No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT140.10395010395010396No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT140.10395010395010396No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG140.10395010395010396No Hit
ATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGCGCCCTTACAC140.10395010395010396No Hit
CTATAGGCGCTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTCA140.10395010395010396No Hit
TTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCA140.10395010395010396No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC140.10395010395010396No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC140.10395010395010396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)