FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13468
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG300.22275022275022277No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT270.20047520047520045No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA260.19305019305019305No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT240.1782001782001782No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC240.1782001782001782No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG220.16335016335016334No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT210.15592515592515593No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC200.1485001485001485No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT190.14107514107514108No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT190.14107514107514108No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC190.14107514107514108No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG190.14107514107514108No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT190.14107514107514108No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT180.13365013365013365No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG170.12622512622512622No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA170.12622512622512622No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA170.12622512622512622No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA160.11880011880011879No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG160.11880011880011879No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT160.11880011880011879No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG160.11880011880011879No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG160.11880011880011879No Hit
ATCACACACCGCACAATCCCCTATCTAGGCCTTCTTACGAGCCAAAACCT150.11137511137511139No Hit
CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCA150.11137511137511139No Hit
CTACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGT150.11137511137511139No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT150.11137511137511139No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA140.10395010395010396No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT140.10395010395010396No Hit
GCCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACT140.10395010395010396No Hit
GGGTGGGACTGTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCT140.10395010395010396No Hit
CTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATCACTC140.10395010395010396No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA140.10395010395010396No Hit
GTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAGGT140.10395010395010396No Hit
GTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAAT140.10395010395010396No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC140.10395010395010396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCC100.008922171133.784384
GTAAGTC100.008922171133.784383
ATGTAAG100.008922171133.784381
GGTTAGT350.00480126257.3361668
Produced by FastQC (version 0.11.5)