FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48107
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC840.1746107635063504No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA720.14966636871972894No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA720.14966636871972894No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT680.14135157045752178No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC560.11640717567090028No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC540.1122497765397967No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC530.11017107697424491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCTA100.007613507141.452182
AGAGACC350.003911488760.6223648
TAAGCAC607.769122E-658.9384083
ATGGCTC400.00663541453.044577
CTAGAGG1059.875866E-441.87307145
AGCACCC750.001659559437.7205855
CTAAGCA1052.1140235E-433.6790922
TAACAGC1700.003521946720.8017927
GGTTAGT1700.003521946720.8017928
Produced by FastQC (version 0.11.5)