FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78226
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1350.17257689259325543No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1300.16618515583054227No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1200.15340168230511594No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1090.13933986142714697No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA990.12655638790172066No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC980.12527804054917802No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG930.11888630378646485No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT910.1163296090813796No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC900.11505126172883695No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT870.11121621967120907No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT860.10993787231866642No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT840.10738117761358117No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA810.10354613555595327No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT790.10098944085086799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCT100.007593469141.633169
AGGGCAA450.001756839274.1888145
CCTTACG502.6287926E-670.816581
AGTGCGG502.6287926E-670.816588
GCTAAAC407.4424766E-570.816583
ACGAGTG502.6287926E-670.816585
GTGCGGC502.6287926E-670.816589
CTAAGTG658.7551365E-568.48197145
GAAATCG500.00266814566.76992145
CCGTGCG451.3333262E-462.9480749
CTAGATA350.003900838360.699934
CCTAGAT350.003900838360.699933
CCAATTC400.006617337553.1124348
CAATTCG400.006617337553.1124349
TATACCG400.006617337553.1124346
TCTATAT400.006617337553.1124343
AAATGCA650.00753921951.361477145
CTCTAGC901.53765E-647.2110562
TAAGTGG1007.3517393E-444.513283145
TCCGTGC658.1897515E-443.5794338
Produced by FastQC (version 0.11.5)