FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33354
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8732.617377226119806No Hit
CGTATATACGTATATACGTATATACGTATATACGTATATAC500.14990705762427295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCA155.590554E-5715.59186145
CTGCGAG158.495933E-4292.2125-129
GCGCACT150.0010082197276.09448120-124
GATCGGC150.0012954037254.08698115-119
AGCAGTA250.0024716302204.25632130-134
GCAGTAG150.0042026043171.88237130-134
ATCTGAA250.004651213165.6567120-124
AGCCCAA200.0071669444143.70493135-139
GTATGAA200.0071669444143.70493135-139
AACAATC150.0078339605139.6972140-144
AGACCCA450.007740981139.14285140-144
CAAACAA450.007740981139.14285140-144
CAACCCT300.008023105138.04724120-124
AAGGTTC200.00397920260.3165177
TAAAGGT200.00397920260.3165175
TATTAAA250.00961944648.2532122
CCATACA500.003606723632.1688082
GGTAAAA653.4591288E-430.9315471
AAAAAAA1600.003873659520.39237140-144
TAGGATT1050.00565017319.14814
Produced by FastQC (version 0.11.5)