FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22029
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN890.40401289209678154No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG270.12256570883834945No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG260.1180262381406328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA200.0072064907143.4656145
TATAAAT251.41821165E-598.6000062
TGCGTAG207.2810723E-492.43758
ATGCGTA207.2810723E-492.43757
ATAAATG207.2810723E-492.43753
AATGCGT207.2810723E-492.43756
AAATGCG207.2810723E-492.43755
TAAATGC350.00669852552.8214344
TATCCAT350.00669852552.8214346
TCCATCC350.00669852552.8214348
CTATCCA350.00669852552.8214345
CCCCATA557.0220005E-444.8181849
CCCCTTA800.004421980630.81251
CAACCAG500.004580295619.893618140-144
TCAACTA651.5234071E-518.59181690-94
GCTTCAG400.0085681917.85761575-79
Produced by FastQC (version 0.11.5)