FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22029
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN890.40401289209678154No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAGGGGTAGCTTACTG280.1271051795360661No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG260.1180262381406328No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA230.10440782604748287No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACA230.10440782604748287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)