FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38230
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA680.1778707821082919No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC650.17002354172116138No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT630.16479204812974102No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC600.15694480774261052No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT580.1517133141511902No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC580.1517133141511902No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC530.1386345801726393No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG510.13340308658121894No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT500.13078733978550877No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC490.1281715929897986No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT460.12032435260266806No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT450.11770860580695788No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC440.11509285901124772No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT420.10986136541982737No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN420.10986136541982737No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC420.10986136541982737No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT410.10724561862411719No Hit
ATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG410.10724561862411719No Hit
GCGGTGGGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGA400.104629871828407No Hit
GCCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACT390.10201412503269684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA353.9269507E-580.460592
CTATACA300.002158694470.4030154
CAACCGT300.002158694470.4030159
GGATTGC300.002158694470.4030159
GAGCTTT500.00279155565.91104145
TAATACG350.003976742360.3454364
TAGGACC400.006745976452.802264
TATACAA400.006745976452.802265
TGCCGCC400.006745976452.802266
GTGATAT400.006745976452.802261
CTTTGCC400.006745976452.802263
GGCCTAC605.6457776E-446.9353451
ACAACCG658.375022E-443.3249328
GGTTATA900.00413901131.2902281
CCTATAC950.005392077429.6433733
AACGCTA254.8239602E-429.31378100-104
TTCTAAA254.901579E-429.23401395-99
ATCCTAT1050.00879011126.8201961
CTAGGGT1050.00879011126.8201964
CCTAGAC1050.00879011126.8201963
Produced by FastQC (version 0.11.5)