FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38230
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA730.1909495160868428No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC680.1778707821082919No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT640.16740779492545121No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC610.1595605545383207No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT600.15694480774261052No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT570.14909756735548No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC560.1464818205597698No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT540.14125032696834947No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT510.13340308658121894No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC480.1255558461940884No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC470.12294009939837823No Hit
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG470.12294009939837823No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG470.12294009939837823No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC450.11770860580695788No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC450.11770860580695788No Hit
GGGTAGGAGTCAGGTAGTTAGTATTAGGAGGGGGGTTGTTAGGGGGTCGG440.11509285901124772No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA440.11509285901124772No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG430.11247711221553754No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC430.11247711221553754No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC420.10986136541982737No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN420.10986136541982737No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG420.10986136541982737No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT400.104629871828407No Hit
TCCCTACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCC390.10201412503269684No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG390.10201412503269684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCA150.0068505104146.39127145
AAGACAA100.007724453140.731326
TGTAGGT100.007724453140.731322
GTGTGCC100.007724453140.731327
GTGCCTT100.007724453140.731329
CTACGTA301.2922646E-7117.276119
ACTACGT353.2389835E-7100.522388
TATGCTG250.00104913984.43885
GTACTGA250.00104913984.43881
ATGCTGT250.00104913984.43886
CCCACCG400.001154128882.34509145
GACTACG451.4455491E-678.184077
ACGGTAC300.002163242870.365668
CTAGACT554.7609265E-663.9687844
AGACTAC554.7609265E-663.9687846
CCACGAC350.003985108360.3134279
CACCGCA350.003985108360.3134279
CTAAGGT350.003985108360.3134273
TAGACTA607.976314E-658.6380545
AGGTGTG502.3017291E-456.292535
Produced by FastQC (version 0.11.5)