FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234775
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA5580.23767436907677567No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG5120.21808114151847516No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT5110.21765520178894684No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC4730.20146949206687254No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT4640.1976360345011181No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC4290.1827281439676286No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC4210.1793206261314024No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT4070.1733574699180066No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT3930.1673943137046108No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT3790.161431157491215No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC3660.15589394100734746No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA3530.15035672452347992No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC3430.1460973272281972No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT3420.14567138749866892No Hit
TCCCTACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCC3340.14226386966244275No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC3260.13885635182621658No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG3230.13757853263763178No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC3150.13417101480140559No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG3010.1282078585880098No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC3000.12778191885848153No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA2950.12565222021084016No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG2930.12480034075178362No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC2870.122244702374614No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT2820.12011500372697263No Hit
GTGTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGG2800.11926312426791608No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA2790.11883718453838783No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT2700.11500372697263338No Hit
GCCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACT2690.11457778724310509No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT2680.11415184751357682No Hit
GTGTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGATGTGTTTAAGTGCT2640.11244808859546374No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG2590.11031838994782239No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC2580.10989245021829411No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2570.10946651048876584No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT2540.10818869130018102No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG2530.10776275157065275No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC2520.1073368118411245No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT2510.10691087211159621No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAGGGGTAGCTTACTG2500.10648493238206794No Hit
GGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAAT2490.10605899265253965No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA2470.10520711319348312No Hit
AGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACTAGTCTCAA2460.10478117346395485No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC2450.10435523373442657No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC2430.10350335427537004No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA2410.10265147481631348No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT2400.10222553508678522No Hit
GGGTGGGACTGTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCT2390.10179959535725695No Hit
ATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACCTA2380.10137365562772867No Hit
CTCCTACCCCTCACAATCATGGCAAGCCAACGCCACTTATCCAGTGAACC2370.10094771589820041No Hit
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG2350.10009583643914385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)