FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234775
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT5380.22915557448621018No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG5180.22063677989564479No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT5180.22063677989564479No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA4910.20913640719838145No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC4270.18187626450857203No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC4030.17165371099989352No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT3930.1673943137046108No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC3910.16654243424555426No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT3810.16228303695027152No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT3800.16185709722074326No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG3610.1537642423597061No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA3450.14694920668725375No Hit
TCCCTACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCC3250.13843041209668833No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG3250.13843041209668833No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT3230.13757853263763178No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC3180.1354488339899904No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA3080.1311894366947077No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG3030.12905973804706633No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC2990.12735597912895325No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG2940.1252262804813119No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC2900.12352252156319882No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG2800.11926312426791608No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC2760.11755936534980299No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT2720.1158556064316899No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC2690.11457778724310509No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC2660.11329996805452029No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAGGGGTAGCTTACTG2640.11244808859546374No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT2600.11074432967735066No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT2580.10989245021829411No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA2540.10818869130018102No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA2540.10818869130018102No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA2470.10520711319348312No Hit
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG2440.10392929400489831No Hit
GTGTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGG2430.10350335427537004No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2410.10265147481631348No Hit
GCCTAGGGTGTTGTGAGTGTAAATTAGTGCGATGAGTAGGGGAAGGGAGC2370.10094771589820041No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT2350.10009583643914385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)