FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158763
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT3210.2021881672681922No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC3130.19714920982848647No Hit
GCCTAGGACTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTT2430.1530583322310614No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA2320.14612976575146602No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT2270.14298041735164993No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT2170.1366817205520178No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAGAGTCATGTCA1910.12030510887297417No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT1810.11400641207334203No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA1740.10959732431359952No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACCATACTCAATTGAT1700.10707784559374665No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGA1680.10581810623382021No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT1650.10392849719393059No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG1590.10014927911415128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCACG756.366463E-1176.038177
TCAGAGT856.6063876E-874.48113145
ATTAGAG1250.074.137213
CTACTGG407.2259005E-571.285789
GAGGCAC1300.071.285787
GTCATAA855.7649067E-662.067608145
AGCCAAG701.6527086E-460.294243145
TTAGATG752.3243639E-456.27463145
GTACAAG651.204048E-554.8352171
CTATAAC400.006458958653.4643362
GGCTCCC400.006458958653.4643367
GCTATAC553.4899093E-451.8442083
CAAGGAA701.8670258E-550.918414
CACATCC859.973537E-750.3193789
TCAGTGA650.00933358648.6992145
CGCATGA1753.6343408E-948.235397145
GGCACCG2000.046.335769
TTAGAGG2000.046.335764
AGCGCTA1153.450874E-545.876057145
AGAGGCA2050.045.205626
Produced by FastQC (version 0.11.5)