FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3698
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT80.2163331530557058No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG80.2163331530557058No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA70.18929150892374255No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA70.18929150892374255No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA60.16224986479177933No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60.16224986479177933No Hit
ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA60.16224986479177933No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT50.1352082206598161No Hit
TCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCC50.1352082206598161No Hit
CATAAATAGTATGGCTTTGAAGAAGGCGTGGGTACAGATGTGCAGGAATG50.1352082206598161No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA50.1352082206598161No Hit
GTGTAAGTTGGGTGCTTTGTGTTAAGCTACACTCTGGTTCGTCCAAGTGC50.1352082206598161No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT50.1352082206598161No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC50.1352082206598161No Hit
CCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTT40.1081665765278529No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG40.1081665765278529No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA40.1081665765278529No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC40.1081665765278529No Hit
CCACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAA40.1081665765278529No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC40.1081665765278529No Hit
CCTGTAATATTGAACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATC40.1081665765278529No Hit
GGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTA40.1081665765278529No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT40.1081665765278529No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA40.1081665765278529No Hit
TAACAGTACATAGCACATAAAGTCATTTACCGTACATAGCACATTACAGT40.1081665765278529No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT40.1081665765278529No Hit
ATGATGGACCATGTAACGAACAATGCTACAGGGATGAATATTATGGAGAA40.1081665765278529No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT40.1081665765278529No Hit
CAACAATCCTAATCCTAATACCAACTATCTCCCTAATTGAAAACAAAATA40.1081665765278529No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT40.1081665765278529No Hit
GTTATGTCCTACAAGCATTAATTAATTAACACACTTCAGTAAGTATGCTC40.1081665765278529No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACA40.1081665765278529No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT40.1081665765278529No Hit
GTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGTCAGGGAGGT40.1081665765278529No Hit
TTATGGTACTGTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATAC40.1081665765278529No Hit
ATTGAACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAG40.1081665765278529No Hit
GTACTGTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATACATAGC40.1081665765278529No Hit
GTAAATAAGGGGTCGTAAGCCTCTGTTGTCAGATTCACAATCTGATGTTT40.1081665765278529No Hit
CTTCAGTAAGTATGCTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA40.1081665765278529No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA40.1081665765278529No Hit
CCCTTAACAGTACATAGCACATAAAGTCATTTACCGTACATAGCACATTA40.1081665765278529No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA40.1081665765278529No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC40.1081665765278529No Hit
CCCATAAATAGGAGAAGGCTTAGAAGAAAACCCCACAAACCCCATTACTA40.1081665765278529No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)