FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3698
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC80.2163331530557058No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT80.2163331530557058No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT70.18929150892374255No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG70.18929150892374255No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA60.16224986479177933No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT50.1352082206598161No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC50.1352082206598161No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG50.1352082206598161No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50.1352082206598161No Hit
CTTCAGTAAGTATGCTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA50.1352082206598161No Hit
GTACTGTTAAGGGTGGGTAGGTTTGTTGGTATCCTAGTGGGTGAGGGGTG50.1352082206598161No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG50.1352082206598161No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC50.1352082206598161No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC40.1081665765278529No Hit
CATTTAATTGTTATTGTTATGTCCTACAAGCATTAATTAATTAACACACT40.1081665765278529No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA40.1081665765278529No Hit
GTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATACATAGCGGTTG40.1081665765278529No Hit
CCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCC40.1081665765278529No Hit
GTACTATTTATACTAAAAGAGTAAGACCCTCATCAATAGATGGAGACATA40.1081665765278529No Hit
GTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTTTATCACTGCTGT40.1081665765278529No Hit
CTACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGT40.1081665765278529No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA40.1081665765278529No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG40.1081665765278529No Hit
GTATAAACTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCC40.1081665765278529No Hit
GTACTGTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATACATAGC40.1081665765278529No Hit
CATCAAAACCCTGCCCCCATGCTTACAAGCAAGTACAGCAATCAACCTTC40.1081665765278529No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC40.1081665765278529No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC40.1081665765278529No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGTGTCT40.1081665765278529No Hit
GTAAAGAATCGTGTGAGGGTGGGACTGTCTACTGAGTAGCCTCCTCAGAT40.1081665765278529No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG40.1081665765278529No Hit
GTACCATAAATACTTGACTACCTGTAGTACATAAAAACTCAACCCACATC40.1081665765278529No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG40.1081665765278529No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG40.1081665765278529No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT40.1081665765278529No Hit
GTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAA40.1081665765278529No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACCATACTCAATTGAT40.1081665765278529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)