FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11811
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGT3242.743205486410973No Hit
ATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATA2331.9727372788078912No Hit
GTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGA1971.6679366692066717No Hit
TACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATG1891.6002032004064008No Hit
GTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGC1771.4986029972059944No Hit
CCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACA1641.388536110405554No Hit
GGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTA1451.2276691220049107No Hit
ATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGG1120.948268563203793No Hit
GCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACA1010.8551350436034206No Hit
ATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAG980.829734992803319No Hit
CCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATC980.829734992803319No Hit
GGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCG940.7958682584031835No Hit
GGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCG840.7112014224028449No Hit
GCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATAC760.6434679536025739No Hit
CATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTA640.5418677504021674No Hit
CATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCA590.4995343324019981No Hit
CCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTT530.448734230801795No Hit
GATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAA510.4318008636017272No Hit
GGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATA510.4318008636017272No Hit
CAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTG480.40640081280162554No Hit
ATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTG440.3725340784014901No Hit
ATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAAT430.3640673948014563No Hit
ACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGC380.32173397680128696No Hit
GTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGC360.3048006096012192No Hit
GTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCC350.29633392600118535No Hit
ATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAAT350.29633392600118535No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN350.29633392600118535No Hit
GGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCA330.27940055880111764No Hit
CCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTA320.2709338752010837No Hit
CTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCT310.26246719160104987No Hit
GCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGA270.2286004572009144No Hit
GAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTA270.2286004572009144No Hit
TCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATAC220.18626703920074505No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG190.16086698840064348No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA180.1524003048006096No Hit
TGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATG180.1524003048006096No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT170.14393362120057573No Hit
ATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGG170.14393362120057573No Hit
ATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATG160.13546693760054185No Hit
TTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATC160.13546693760054185No Hit
TGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGG150.12700025400050802No Hit
CCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACAT150.12700025400050802No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC140.11853357040047413No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC140.11853357040047413No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT120.10160020320040639No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG120.10160020320040639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)