FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61305
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1150.18758665687953674No Hit
GTCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT1070.17453715031400377No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAGAGTCATGTCA790.12886387733463828No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA760.12397031237256341No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCTACCG760.12397031237256341No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC750.12233912405187179No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC720.11744555908979692No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT690.11255199412772204No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT690.11255199412772204No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA650.10602724084495556No Hit
GCGGTAGGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGA650.10602724084495556No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT640.10439605252426393No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA640.10439605252426393No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACCATACTCAATTGAT630.10276486420357231No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG620.10113367588288066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA100.0075873225141.646826
CTTACGT100.0075873225141.646823
AACCACG257.2075873E-6113.317467
GACCATA353.1391755E-7101.17631
AGCCTTA301.7830107E-594.431216
CCGTGCG250.001025455584.988099
TATAGCC353.831298E-580.941047
CCTACAT450.001629993175.569626145
TATGCGA450.001629993175.569626145
GGATATT300.001674093975.11202135-139
TGTATAT407.426158E-570.823413
CCATGTT500.002475672968.012665145
TTGAACC350.00329121663.340668115-119
GCCTTAC451.3304106E-462.9541447
ATATAGC451.3304106E-462.9541446
ATCTCAG350.003895346860.705782
ACGCTCA350.003895346860.705789
TCTCAGA350.003895346860.705783
GACGCTC350.003895346860.705788
ACCTATG607.7313125E-659.019517
Produced by FastQC (version 0.11.5)