FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46209
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2460.5323638252288515No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG600.1298448354216711No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA500.10820402951805924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTAT300.004448087458.681727
TATAGAG401.8783429E-458.681725
TAGAGAC350.00818499350.298627
TGCTTGG350.00818499350.298627
GCTTGGT350.00818499350.298628
CTTGGTT350.00818499350.298629
AGAGACC350.00818499350.298628
ATAGAGA505.651996E-446.9453776
AAAAGGT456.773058E-445.265766130-134
GTCGTAA600.00138671639.1211471
TATTATG851.8280096E-434.5186582
AGGTTGG500.00377826832.883232145
TTATGCT902.5572715E-432.6009564
TATGCTT1004.7428237E-429.3408625
TTTTTAG400.00701536928.131716100-104
ATATTAT1250.001744991523.4726871
ATTATGC1300.002192067722.5698913
TAAGGGG1600.00729604218.3380375
Produced by FastQC (version 0.11.5)