FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89506
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1520.16982101758541326No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1220.13630371148302908No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT1130.12624851965231382No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA1120.12513127611556768No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1110.12401403257882153No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGCTTTTTGGAAAGTCATGTCA1020.11395884074810628No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG1020.11395884074810628No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT970.10837262306437558No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC970.10837262306437558No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA950.10613813599088329No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT920.10278640538064487No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA920.10278640538064487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)