FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20118
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN420.20876826722338201No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT350.17397355601948505No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA310.15409086390297247No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA280.13917884481558804No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC280.13917884481558804No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG280.13917884481558804No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT240.11929615269907547No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT230.11432547966994731No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG230.11432547966994731No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT220.10935480664081916No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG220.10935480664081916No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT210.10438413361169101No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA210.10438413361169101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTTT150.0044220258168.97865145
ACTACTT150.0044220258168.97865145
GATGGCG300.003129158263.9975137
ATGGCGG300.003129158263.9975138
TGTAGAG400.00976825147.9981352
TATACTA1156.3418417E-427.8250065
Produced by FastQC (version 0.11.5)