FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34380
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1630.4741128563118092No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG740.2152414194299011No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT640.18615474112856312No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG520.15125072716695753No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT440.12798138452588714No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA420.12216404886561956No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA380.11052937754508435No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG380.11052937754508435No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT380.11052937754508435No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC370.10762070971495055No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG370.10762070971495055No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC360.10471204188481677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATTG200.0047960067164.58472145
AGAGGCT250.00222197269.876313
CAGAGGC250.00222197269.876312
GCTTACG250.00222197269.876317
ACAGAGG300.004576170458.2302631
TCCTAAC350.00842032449.9116551
GGCTTAC350.00842032449.9116556
TAACACT505.8500917E-446.584214
TGATTTA707.6278586E-540.013565140-144
CCTAAAC650.002126402735.8340073
CTAATCT700.00305838433.2744374
TACTAAT1051.6716787E-533.2744372
TTAACAC750.00428777531.056143
GTGAGAG300.00874677726.889114125-129
ACTAATC1751.993791E-523.2921073
Produced by FastQC (version 0.11.5)