FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11368
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC290.25510204081632654No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT240.211118930330753No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT230.20232230823363828No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA210.18472906403940886No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG190.16713581984517945No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG190.16713581984517945No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC190.16713581984517945No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT180.15833919774806474No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT170.14954257565095003No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG170.14954257565095003No Hit
TTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCA170.14954257565095003No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG160.14074595355383532No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT160.14074595355383532No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA160.14074595355383532No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG160.14074595355383532No Hit
ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA160.14074595355383532No Hit
GTACTATTTATACTAAAAGAGTAAGACCCTCATCAATAGATGGAGACATA150.1319493314567206No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC150.1319493314567206No Hit
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG140.12315270935960591No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC140.12315270935960591No Hit
TTCCTACACATCGGACGAGGCCTATATTACGGATCATTTCTCTACTCAGA130.1143560872624912No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC130.1143560872624912No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT130.1143560872624912No Hit
GTCCTTGTAGTATAAACTAATACACCAGTCTTGTAAACCGGAGATGAAAA130.1143560872624912No Hit
TCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCC120.1055594651653765No Hit
TTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAATT120.1055594651653765No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA120.1055594651653765No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT120.1055594651653765No Hit
CCCTTAACAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTA120.1055594651653765No Hit
GTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGT120.1055594651653765No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA120.1055594651653765No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA120.1055594651653765No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT120.1055594651653765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)