FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11368
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT290.25510204081632654No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG260.2287121745249824No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC210.18472906403940886No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG190.16713581984517945No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC180.15833919774806474No Hit
CCCTTAACAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTA170.14954257565095003No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT170.14954257565095003No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC160.14074595355383532No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG160.14074595355383532No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG150.1319493314567206No Hit
CTACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGT150.1319493314567206No Hit
TTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCA140.12315270935960591No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA140.12315270935960591No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG130.1143560872624912No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT130.1143560872624912No Hit
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG130.1143560872624912No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC130.1143560872624912No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT130.1143560872624912No Hit
ATATATCACAGTGAGAATTCTATGATGGACCATGTAACGAACAATGCTAC130.1143560872624912No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA120.1055594651653765No Hit
TAGTTATGTCATCCCTCTTATTAATCATCATCCTAGCCCTAAGTCTGGCC120.1055594651653765No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG120.1055594651653765No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA120.1055594651653765No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG120.1055594651653765No Hit
GTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCA120.1055594651653765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)