FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9520
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310.32563025210084034No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG200.21008403361344538No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC190.1995798319327731No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT190.1995798319327731No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG190.1995798319327731No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA180.18907563025210083No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG180.18907563025210083No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC150.15756302521008403No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG140.14705882352941177No Hit
TTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCA130.13655462184873948No Hit
CTTCAGTAAGTATGCTCGTCTGTAATATTGAACGTAGGTGCGATAAATAA130.13655462184873948No Hit
ATATTGTACAGTACCATAAATACTTGACTACCTGTAGTACATAAAAACTC120.12605042016806722No Hit
GTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCCTCAGAATG120.12605042016806722No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC120.12605042016806722No Hit
CTCTTCAATCAGCCACATGGCCCTCGTAGTAACAGCCATTCTCATCCAAA110.11554621848739496No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG110.11554621848739496No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT110.11554621848739496No Hit
CCCTTAACAGTACATAGCACATAAAGTCATTTACCGTACATAGCACATTA110.11554621848739496No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA100.10504201680672269No Hit
GGCTAAGGTTGTCTGGTAGTAAGGTGGAGTGGGTTTGGGGCTAGGTTTAG100.10504201680672269No Hit
CTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACAAGA100.10504201680672269No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGAGGCTCACTCAC100.10504201680672269No Hit
GTCCTTGTAGTATAAACTAATACACCAGTCTTGTAAACCGGAGATGAAAA100.10504201680672269No Hit
TCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCC100.10504201680672269No Hit
ATTCTCACCAGACCTCCTAGGCGACCCAGACAATTATACCCTAGCCAACC100.10504201680672269No Hit
GTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGG100.10504201680672269No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG100.10504201680672269No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG100.10504201680672269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)