FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9520
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN300.31512605042016806No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT180.18907563025210083No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT170.17857142857142858No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC150.15756302521008403No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT150.15756302521008403No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC140.14705882352941177No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG140.14705882352941177No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG130.13655462184873948No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC130.13655462184873948No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACATTCTCCTGA120.12605042016806722No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC120.12605042016806722No Hit
GTGTAGTAAGGGTGGAAGGTGATTTTATCGGAATGGGAGGTGATTCCTAG110.11554621848739496No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG110.11554621848739496No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA110.11554621848739496No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA100.10504201680672269No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA100.10504201680672269No Hit
ATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTT100.10504201680672269No Hit
CTGTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATAT100.10504201680672269No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAGTCTTCTTACTCAT100.10504201680672269No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT100.10504201680672269No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT100.10504201680672269No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG100.10504201680672269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)