FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76861
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1550.20166274183265895No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1140.1483196939930524No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG1110.14441654415112995No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT1080.14051339430920753No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA1080.14051339430920753No Hit
GGATTAGACTGAGCCGAATTGGTATATAGTTTAAACAAAACGAATGATTT970.1262018448888253No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT960.1249007949415178No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA960.1249007949415178No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT840.10928819557382809No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC820.10668609567921312No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA810.10538504573190564No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG790.1027829458372907No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA770.10018084594267573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)