FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76861
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1870.2432963401464982No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1260.1639322933607421No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT1200.15612599367689725No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT1050.1366102444672851No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA1020.13270709462536268No Hit
GGATTAGACTGAGCCGAATTGGTATATAGTTTAAACAAAACGAATGATTT960.1249007949415178No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG940.12229869504690284No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA900.11709449525767295No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG850.11058924552113557No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA840.10928819557382809No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC820.10668609567921312No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA790.1027829458372907No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC770.10018084594267573No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT770.10018084594267573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCA300.002103231670.9358759
AGTGGTG500.002645247666.91351145
GCCGTTG350.003125579364.187035135-139
GTTAACA350.003874676360.8021741
CTCCATG400.00657301753.2019049
GTAAAAC400.00657301753.2019049
CCATGCA553.5640856E-451.5897259
TCACACA658.121313E-443.6528434
TATAGAG908.4354346E-539.4088177
ACAGCTA1459.691488E-538.45604145
GTATAGA951.15980874E-437.3346676
ATAGAGA1201.097315E-535.4679378
CTTTAGT800.002253717535.4679371
TAGAGAG1102.7452357E-432.243589
GTACTGA900.004014126531.5270541
TATAACC900.004014126531.5270543
CTATATC950.005229518329.8677354
TAGTCAA1550.006246966328.780005145
GAGCCCT1255.8058463E-428.3743486
AAAGGAG1255.8058463E-428.3743485
Produced by FastQC (version 0.11.5)