FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA70.2722676001555815No Hit
GGCTAAGGTTGTCTGGTAGTAAGGTGGAGTGGGTTTGGGGCTAGGTTTAG70.2722676001555815No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG70.2722676001555815No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA60.2333722287047841No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG60.2333722287047841No Hit
GGCTTTAACATATCTGAATACACAATAGCTAAGACCCAAACTGGGATTAG50.19447685725398678No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT50.19447685725398678No Hit
GGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTA50.19447685725398678No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT50.19447685725398678No Hit
GTACATAGCACATAAAGTCATTTACCGTACATAGCACATTACAGTCAAAT50.19447685725398678No Hit
GTCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT50.19447685725398678No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT40.15558148580318942No Hit
TCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCC40.15558148580318942No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA40.15558148580318942No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT40.15558148580318942No Hit
ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA40.15558148580318942No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT40.15558148580318942No Hit
GTACAGTACCATAAATACTTGACTACCTGTAGTACATAAAAACTCAACCC30.11668611435239205No Hit
CTGCGACATAGGGTGCTCCGGCTCCAGCGTCCCGCAATGCTATCGCGTGC30.11668611435239205No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC30.11668611435239205No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC30.11668611435239205No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT30.11668611435239205No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATTACCAC30.11668611435239205No Hit
ATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTC30.11668611435239205No Hit
CGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGC30.11668611435239205No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG30.11668611435239205No Hit
GTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGATGCATGAGTA30.11668611435239205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.11668611435239205No Hit
ACCATAAATACTTGACTACCTGTAGTACATAAAAACTCAACCCACATCAA30.11668611435239205No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT30.11668611435239205No Hit
CTATAGGCGCTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTCA30.11668611435239205No Hit
GTACCATAAATACTTGACTACCTGTAGTACATAAAAACTCAACCCACATC30.11668611435239205No Hit
AGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAA30.11668611435239205No Hit
TAACAGTACATAGCACATAAAGTCATTTACCGTACATAGCACATTACAGT30.11668611435239205No Hit
GTGTAGGGAGCTGAATAATTTATGAAGGAGAGGGGTCAGGGTTGATTCGG30.11668611435239205No Hit
CAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAA30.11668611435239205No Hit
GAGCTGGGTTGTTTGGGTTGTGGCTCAGTGTCAGTTCGAGATAATAACTT30.11668611435239205No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC30.11668611435239205No Hit
GATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCG30.11668611435239205No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA30.11668611435239205No Hit
GTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGG30.11668611435239205No Hit
GTAAAAAACTCCAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACAT30.11668611435239205No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG30.11668611435239205No Hit
TCAATATTCTTTATCTGCCTCTTCCTACACATCGGGCGAGGCCTATATTA30.11668611435239205No Hit
GAGTAAGACCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCAC30.11668611435239205No Hit
GCGGTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGA30.11668611435239205No Hit
GTACTGTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATACATAGC30.11668611435239205No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC30.11668611435239205No Hit
GGGTGGGACTGTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCT30.11668611435239205No Hit
AGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTC30.11668611435239205No Hit
GCTAAATAGGTTGTTGTTGATTTGGTTAAAAAATAGTAGGGGGATGATGC30.11668611435239205No Hit
TGTTAGGGTTGTACGGTAGAACTGCTATTATTCATCCTATGTGGGTAATT30.11668611435239205No Hit
CACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCAAAGCCATACT30.11668611435239205No Hit
GTCGAAGCCCCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAA30.11668611435239205No Hit
TGTAAATATAGTTTAACCAAAACATCAGATTGTGAATCTGACAACAGAGG30.11668611435239205No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACAC30.11668611435239205No Hit
CTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATCACTC30.11668611435239205No Hit
CAGATAGGGGTCCCTTGACCACCATCCTCCGTGAAATCAATATCCCGCAC30.11668611435239205No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA30.11668611435239205No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC30.11668611435239205No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC30.11668611435239205No Hit
ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCCCGCA30.11668611435239205No Hit
GGTGGAAGGTGATTTTATCGGAATGGGAGGTGATTCCTAGGGGGTTGTTT30.11668611435239205No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC30.11668611435239205No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)