FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9912
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT200.20177562550443903No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG180.18159806295399517No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA170.1715092816787732No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC170.1715092816787732No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA150.1513317191283293No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG130.1311541565778854No Hit
TAACAGTACATAGCACATAAAGCCATTTACCGTACATAGCACATTACAGT130.1311541565778854No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA130.1311541565778854No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA120.12106537530266344No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT120.12106537530266344No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC110.11097659402744149No Hit
CCCTTAACAGTACATAGCACATAAAGCCATTTACCGTACATAGCACATTA110.11097659402744149No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC110.11097659402744149No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG110.11097659402744149No Hit
GTGTAAGTTGGGTGCTTTGTGTTAAGCTACACTCTGGTTCGTCCAAGTGC110.11097659402744149No Hit
TCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCC100.10088781275221952No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT100.10088781275221952No Hit
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG100.10088781275221952No Hit
ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGG100.10088781275221952No Hit
GTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATACATAGCGGTTG100.10088781275221952No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT100.10088781275221952No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT100.10088781275221952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTG100.002137486214.83722145
TACAATA250.001048479283.981822
CTAGGAG300.002161998869.984853
ACCCTAA300.002161998869.984851
ACAATAT300.002161998869.984853
CCCTAAC350.003983031559.9870152
Produced by FastQC (version 0.11.5)