EGAF00005577382 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005577382
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68990
Sequences flagged as poor quality	0
Sequence length	35-151
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC	120	0.1739382519205682	No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA	105	0.15219597043049718	No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT	103	0.14929699956515435	No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC	94	0.13625163067111173	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG	94	0.13625163067111173	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC	93	0.13480214523844036	No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA	86	0.12465574720974054	No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT	86	0.12465574720974054	No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT	84	0.12175677634439774	No Hit
GCGGTAGGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGA	81	0.11740832004638355	No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT	79	0.11450934918104073	No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC	79	0.11450934918104073	No Hit
AGTCTTTGCCGCCTGCGAAGCAGCGGTAGGCCTAGCCCTACTAGTCTCAA	78	0.11305986374836932	No Hit
GTCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT	78	0.11305986374836932	No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT	76	0.11016089288302654	No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC	75	0.10871140745035512	No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT	74	0.10726192201768373	No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT	74	0.10726192201768373	No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGAGGCTCACTCAC	72	0.10436295115234093	No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC	72	0.10436295115234093	No Hit
GCCTAGGACTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTT	70	0.10146398028699811	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA	69	0.1000144948543267	No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAGTCTTCTTACTCAT	69	0.1000144948543267	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATAA	10	0.0076455274	141.29774	9
CTTTGCC	20	4.2678713E-4	105.973305	4
TCTTTGC	20	4.2678713E-4	105.973305	3
GCGCGTA	30	0.0015884683	76.12167	140-144
AGTCTTT	30	0.00213638	70.64887	1
TTCATAC	55	4.688789E-6	64.22625	3
ATACAGG	55	4.688789E-6	64.22625	6
ATCCTGA	35	0.003935661	60.556175	6
AGCCTTA	35	0.003935661	60.556175	6
CAATAGT	35	0.003935661	60.556175	4
AGGCTAT	35	0.003935661	60.556175	6
CCTAACG	60	0.005215375	56.36356	145
GTACAAC	40	0.006676332	52.986652	1
GTGTGCA	40	0.006676332	52.986652	9
GCCCTGT	40	0.006676332	52.986652	8
CCGCCCC	40	0.006676332	52.986652	9
CCTGAGG	40	0.006676332	52.986652	8
CGCGTAC	30	3.867851E-4	50.767647	140-144
ATTCATA	75	2.9429077E-5	47.09925	2
CATACAG	80	4.3085485E-5	44.155544	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content