FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2675
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100.3738317757009346No Hit
CTTTAGTAGGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA60.22429906542056074No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG50.1869158878504673No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC50.1869158878504673No Hit
CACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCC50.1869158878504673No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG50.1869158878504673No Hit
CTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAA40.14953271028037382No Hit
GTTCCTGCTCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGA40.14953271028037382No Hit
ACCATGTACTGTTAAGGATAGGTAGGTTTGTTGATATCCTAGTGGGTGAG40.14953271028037382No Hit
CTTTAATAGGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA40.14953271028037382No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT40.14953271028037382No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA40.14953271028037382No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC40.14953271028037382No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC40.14953271028037382No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC40.14953271028037382No Hit
GTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAACG40.14953271028037382No Hit
GTGTAAGTTGGGTGCTTTGTGTTAAGCTACACTCTGGTTCGTCCAAGTGC40.14953271028037382No Hit
AACTAGGACTCATAATAGTTACAATCGGCATCAACCAACCACACCTAGCA40.14953271028037382No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT30.11214953271028037No Hit
GTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATT30.11214953271028037No Hit
CTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGA30.11214953271028037No Hit
GCTAAAGGTTAATCACTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGC30.11214953271028037No Hit
CGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGC30.11214953271028037No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA30.11214953271028037No Hit
GGATGATGGCGGGCAGGATAGTTCAGACGGTCTCTATTTCCTGAGCGTCT30.11214953271028037No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA30.11214953271028037No Hit
GTTTAGCTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTAAGTGTAAGTTG30.11214953271028037No Hit
GTCCTTGTAGTATAAACTAATACACCAGTCTTGTAAACCGGAGATGAAAA30.11214953271028037No Hit
TCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAAC30.11214953271028037No Hit
GAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGG30.11214953271028037No Hit
ATGTACTGTTAAGGATAGGTAGGTTTGTTGATATCCTAGTGGGTGAGGGG30.11214953271028037No Hit
ACACATAATGACCCACCAATCGCATGCCTATCATATAGTAAAACCCAGCC30.11214953271028037No Hit
TTGTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGC30.11214953271028037No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA30.11214953271028037No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT30.11214953271028037No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC30.11214953271028037No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT30.11214953271028037No Hit
CCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCAT30.11214953271028037No Hit
ATATGATAGGCATGCGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAG30.11214953271028037No Hit
GGTGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATATATGGT30.11214953271028037No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACA30.11214953271028037No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA30.11214953271028037No Hit
GTATAAACTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCC30.11214953271028037No Hit
ATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCC30.11214953271028037No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG30.11214953271028037No Hit
CCTCTTTACAGTGAAATGCCCCAACTAAATACTACCGTATGGCCCACCAT30.11214953271028037No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC30.11214953271028037No Hit
GTTAAGGATAGGTAGGTTTGTTGATATCCTAGTGGGTGAGGGGTGGCTTT30.11214953271028037No Hit
CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCA30.11214953271028037No Hit
ACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAA30.11214953271028037No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC30.11214953271028037No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA30.11214953271028037No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC30.11214953271028037No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT30.11214953271028037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAGT200.008766744129.84615145
CTACAAA100.009864793127.396229
TCCTTGT100.009864793127.396221
TTGTACT100.009864793127.396224
CCTTGTA100.009864793127.396222
AAGGCTA100.009864793127.396225
AGGCTAC100.009864793127.396226
TGAAGGC100.009864793127.396223
CTTGTAC100.009864793127.396223
TGTACTA100.009864793127.396225
TACTATC100.009864793127.396227
GAAGGCT100.009864793127.396224
Produced by FastQC (version 0.11.5)