FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16928
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1490.8801984877126655No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG230.1358695652173913No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA220.12996219281663515No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC220.12996219281663515No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA200.11814744801512288No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA190.11224007561436673No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC180.10633270321361059No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT180.10633270321361059No Hit
ATTACTATCCATCCTCATTCTAGCAATAATCCCCATCCTCCATATATCCA170.10042533081285444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAA250.009025004132.6087145
CTATGGT250.0024417868.080364
TAATATA200.004939069557.07335380-84
TCGCTCA350.00925076248.6288269
ATCGCTC350.00925076248.6288268
Produced by FastQC (version 0.11.5)