FastQCFastQC Report
Wed 8 May 2024
EGAF00005577396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196825
Total Bases29.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4600.23371014860917058No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA4420.22456496888098565No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC4020.2042423472627969No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT3940.20017782293915917No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT3890.19763749523688556No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC3830.19458910199415724No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC3560.18087133240187983No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT3410.17325034929505906No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT3380.1717261526736949No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGGGTGATAT3270.166137431728693No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA3160.1605487107836911No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC3050.1549599898386892No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC2850.1447986790295948No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG2800.14225835132732123No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT2740.1392099580845929No Hit
TCCCTACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCC2560.13006477835640798No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC2510.1275244506541344No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT2480.12600025403277024No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA2400.12193572970913248No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG2350.11939540200685889No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC2340.11888733646640416No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG2330.11837927092594944No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG2290.11634700876413057No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2280.11583894322367584No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA2270.11533087768322114No Hit
CTCCTACCCCTCACAATCATGGCAAGCCAACGCCACTTATCCAGCGAACC2250.1143147466023117No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA2250.1143147466023117No Hit
AGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACTAGTCTCAA2250.1143147466023117No Hit
GCCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACT2240.11380668106185697No Hit
CTCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGATCAACCTCACCAC2220.11279054998094755No Hit
GTGTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGATGTGTTTAAGTGCT2200.1117744189000381No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG2200.1117744189000381No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT2180.11075828781912866No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA2150.1092340911977645No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG2150.1092340911977645No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT2130.10821796011685507No Hit
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG2100.10669376349549092No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC2090.1061856979550362No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC2080.10567763241458147No Hit
GGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTA2040.1036453702527626No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT2030.10313730471230788No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC2030.10313730471230788No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC2020.10262923917185318No Hit
ATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG2010.10212117363139846No Hit
GATTAGTATAGAGAGGTAGAGTTTTTTTCGTGATAGTGGTTCGCTGGATA1990.10110504255048901No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT1990.10110504255048901No Hit
GAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA1970.10008891146957959No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC1970.10008891146957959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)