FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196825
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4570.23218595198780642No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA4420.22456496888098565No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC4010.2037342817223422No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT3920.19916169185824972No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT3890.19763749523688556No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC3810.19357297091324782No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC3540.1798552013209704No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT3410.17325034929505906No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT3380.1717261526736949No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGGGTGATAT3250.16512130064778358No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA3130.15902451416232694No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC3010.15292772767687032No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC2840.1442906134891401No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG2800.14225835132732123No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT2720.1381938270036835No Hit
TCCCTACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCC2550.12955671281595327No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC2510.1275244506541344No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT2450.12447605741140606No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA2400.12193572970913248No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG2350.11939540200685889No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG2330.11837927092594944No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC2330.11837927092594944No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG2270.11533087768322114No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2270.11533087768322114No Hit
CTCCTACCCCTCACAATCATGGCAAGCCAACGCCACTTATCCAGCGAACC2250.1143147466023117No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA2250.1143147466023117No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA2240.11380668106185697No Hit
GCCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACT2240.11380668106185697No Hit
AGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACTAGTCTCAA2210.11228248444049282No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG2200.1117744189000381No Hit
CTCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGATCAACCTCACCAC2190.11126635335958338No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT2180.11075828781912866No Hit
GTGTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGATGTGTTTAAGTGCT2170.11025022227867395No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA2150.1092340911977645No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG2150.1092340911977645No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT2110.10720182903594565No Hit
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG2090.1061856979550362No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC2080.10567763241458147No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC2070.10516956687412678No Hit
GGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTA2040.1036453702527626No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC2030.10313730471230788No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT2020.10262923917185318No Hit
ATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG2000.10161310809094373No Hit
GATTAGTATAGAGAGGTAGAGTTTTTTTCGTGATAGTGGTTCGCTGGATA1990.10110504255048901No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC1990.10110504255048901No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT1980.10059697701003428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAGT150.007397964142.91103145
CACTGGT300.002059379171.355617
AGGACGG350.003793979861.161965
CTGTCTA1051.2678356E-954.3661849
TAGGACG400.006436281453.5167124
CACCAGC400.006436281453.5167127
GGGACTG953.5392077E-852.5778245
ACTGTCT1005.3019903E-849.9489368
GACTGTC1005.3019903E-849.9489367
GGTGGGC1152.8394425E-949.6386873
CTTTGCC605.3381035E-447.570414
CGGTGGG1204.14002E-947.570412
TGCCGCC605.3381035E-447.570417
CCGTGCG1950.047.5704089
TCCGTGC1950.047.5704088
AGGAGCC1700.046.1712847
CGCATGA1452.3766606E-644.351704145
GGTGGGA1151.5919431E-743.4338532
GTAAGAG1151.5919431E-743.4338539
CCTAACG2001.033186E-842.87331145
Produced by FastQC (version 0.11.5)