FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCATACTCTTCAATCAGCCACATAGCCCTCGCAGTAACAGCCAATCTC15.0No Hit
GAAAAAGTCTTTAACTCCACCATTAGCACCCAAAGCTAAGATTCTAATTT15.0No Hit
GGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCG15.0No Hit
ATTATTAGTAGCAAGCCCGAGAAGGGTTTCACTATTAAAATTTAAGCTGA15.0No Hit
GGGCTAGGCTGGAGTGGCAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAA15.0No Hit
CTTGGGTGGTACCCAAATCTGCTTCCCCATGAAAGAACAGAGAAT15.0No Hit
CATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGA15.0No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG15.0No Hit
ATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATTGACCC15.0No Hit
CCCAAAGGTTAGTCACGGCCGTTGCGTAGGCCGCGGTGGCTAGACAAAGC15.0No Hit
CCATCAGAGGATCGCAGGCTAACCTATTATCGTTCTTCGCATTCTATTCT15.0No Hit
CGTTTAGTGAGGGAGAGACTTGGTATATGATTGAGATGGGGGCTAGTTTT15.0No Hit
TGTAAGAATGTAGCGCAGCGTTGAGTGCACGCCCTGCGACCCGGGCGCGG15.0No Hit
AAATATCACTCTCCTACTTACAGGACTCAACATACTAGTCACAGCCCTATACTCCCTCTACATATTTACC15.0No Hit
CTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCA15.0No Hit
ATCCACCTCAACTGCCTGCTATGATGGATAAGATTGAGAGAGTGAGGAGA15.0No Hit
ATATAATTCCTACGCCCTCTCAGCCGATGAACAGTTGGAATAGGTTGTTA15.0No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15.0No Hit
GAACGGGGATGCTTGCATGTGTAATCTTACTAAGAGC15.0No Hit
ATCTAACTCCTCCTATTTACCCAATGCGGCTCCTCGAGGAGTACGCTAAT15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)