FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121365
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA3300.27190705722407615No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2950.24306842994273473No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT2480.20434227330779056No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC2370.19527870473365466No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC2340.19280682239525399No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC2330.1919828616157871No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT2230.1837432538211181No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG2120.17467968524698224No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT2030.16726403823178018No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT2010.16561611667284637No Hit
TCCCCACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCC1920.15820046965764428No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC1890.1557285873192436No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC1850.15243274420137604No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1840.15160878342190912No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC1740.14336917562724014No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC1610.13265768549417048No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1600.1318337247147036No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT1590.1310097639352367No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG1550.1277139208173691No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT1500.1235941169200346No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT1500.1235941169200346No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC1500.1235941169200346No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1460.12029827380216702No Hit
ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA1430.11782639146376633No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC1420.11700243068429944No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT1400.11535450912536563No Hit
GCCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACT1400.11535450912536563No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC1390.11453054834589874No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG1380.11370658756643184No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA1370.11288262678696492No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1370.11288262678696492No Hit
GGGTGGGACTGTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCT1360.11205866600749803No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC1360.11205866600749803No Hit
AGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACTAGTCTCAA1360.11205866600749803No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG1340.11041074444856425No Hit
GATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA1320.10876282288963045No Hit
GTGTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGG1300.10711490133069666No Hit
GTGTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGATGTGTTTAAGTGCT1290.10629094055122977No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA1290.10629094055122977No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1260.10381905821282907No Hit
GAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA1260.10381905821282907No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT1260.10381905821282907No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAAC1250.10299509743336217No Hit
ATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACCTA1230.10134717587442837No Hit
GACCTAACCTGACTAGAAAAGCTATTACCTAAAACAATTTCACAGCACCA1220.10052321509496148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCC204.0845884E-4107.199224
TCTTTGC259.916632E-485.759373
TGCCGCC259.916632E-485.759377
CGTACTT353.6779456E-581.675595
TTGCCGT353.6779456E-581.675591
TGCCGTA353.6779456E-581.675592
GCCGTAC407.129208E-571.466153
GGTAGGG608.8060915E-871.466142
AAGCGAG1050.068.062999
CGCATGA1501.0913936E-1164.06823145
CCTGTTT500.003149909464.06823145
CTAGATA808.969437E-962.532884
CCTAGAT808.969437E-962.532883
CACCAGC350.00376729461.2566957
GAAGCGA1154.5474735E-1155.9300238
TATACTC1152.7866918E-949.7155765
GTCATAT1301.1441734E-649.283253145
GGCGTGA1301.1441734E-649.283253145
TCACCGG1556.924529E-848.223396145
CCTGGGG752.7620266E-547.6440963
Produced by FastQC (version 0.11.5)