FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121365
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT2670.2199975281176616No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT2610.21505376344086022No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2590.21340584188192643No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC2370.19527870473365466No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA2300.1895109792773864No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT2140.17632760680591603No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC2110.17385572446751535No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC2100.17303176368804846No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG2050.16891195979071397No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT2000.16479215589337948No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC2000.16479215589337948No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1990.16396819511391258No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1950.160672351996045No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC1690.13924937172990565No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG1650.13595352861203808No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA1610.13265768549417048No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1580.13018580315576977No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG1550.1277139208173691No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC1510.12441807769950151No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC1480.12194619536110081No Hit
GCCTAGGGTGTTGTGAGTGTAAATTAGTGCGATGAGTAGGGGAAGGGAGC1450.11947431302270012No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAAC1430.11782639146376633No Hit
GTGTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGG1400.11535450912536563No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT1370.11288262678696492No Hit
TCCCCACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCC1330.10958678366909735No Hit
GAATAGTCCTGTGGTGATTTGGAGGATCAGGCAGGCGCCAAGGAGTGAGC1320.10876282288963045No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG1310.10793886211016356No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG1290.10629094055122977No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT1270.10464301899229596No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC1250.10299509743336217No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1240.10217113665389528No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT1240.10217113665389528No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT1220.10052321509496148No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1220.10052321509496148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATC100.007392079142.944677
CTACGTG100.007392079142.944674
ACGTGAT100.007392079142.944676
TACGTGA100.007392079142.944675
GGCTAAA100.007392079142.944671
CTAGGGA303.922914E-4108.25891145
GTGGGCC406.624541E-789.3404164
CGGTGGG451.3349854E-679.413712
ACCTGCA300.002044166271.472348
CCTGCAT300.002044166271.472349
GGTGGGC502.4970468E-671.4723363
CCAGCCT451.2767164E-463.5309689
CACCAGC451.2767164E-463.5309687
GACTACA1150.062.1498571
GCACACA955.11136E-1060.187231
CCACGAC1201.8189894E-1259.5602849
CGCATGA1507.348717E-1057.738087145
GGTCATT1559.822543E-1055.87557145
CTCCAGT802.811993E-454.12945145
ATTAGAG400.006388898553.604253
Produced by FastQC (version 0.11.5)