FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132028
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA2950.22343745266155665No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC2540.1923834338170691No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT2450.1855666979731572No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC2440.18480928287938922No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC2430.18405186778562124No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2370.1795073772230133No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT2200.1666313206289575No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT2010.1522404338473657No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1840.1393643772533099No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1830.1386069621595419No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT1810.13709213197200595No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT1810.13709213197200595No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT1810.13709213197200595No Hit
GCCATACACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA1740.13179022631563003No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC1730.13103281122186203No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT1720.13027539612809405No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC1650.12497349047171812No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT1630.12345866028418213No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA1540.11664192444027024No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG1520.11512709425273426No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT1510.11436967915896627No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1460.11058260369012633No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG1450.10982518859635834No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC1440.10906777350259036No Hit
GTGTATGGCTAGGAATAGTCCTGTGGTGATTTGGAGGATCAGGCAGGCGC1430.10831035840882237No Hit
CTGTAGCATTGTTCGTTACATGGTCAATCATAGAATTCTCACTGTGATAT1430.10831035840882237No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC1420.10755294331505438No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG1420.10755294331505438No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1390.10528069803375043No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC1360.10300845275244645No Hit
GTGTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGATGTGTTTAAGTGCT1350.10225103765867846No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG1340.10149362256491049No Hit
GTCTAGGGCTGTTAGAAGTCCTAGGAAAGTGACAGCGAGGGCTGTGAGTT1330.1007362074711425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGGC202.3099983E-6142.570512
CTGGCCA202.3099983E-6142.570514
CTCCTGG257.008577E-6114.0564041
ACTCCCG301.7338227E-595.047018
TGGCCAC353.7256985E-581.4688645
GTACTCC353.7256985E-581.4688646
CGAATCA906.776099E-874.181656145
ACTGATC300.002065938271.285268
CACTGGT300.002065938271.285267
TACTGAT300.002065938271.285267
ACTACGC300.002065938271.285268
TATACTC1152.8521754E-949.5897455
GTTCGGG605.357661E-447.5235061
CCTGGCC752.8050832E-547.5235023
CCGTGCG952.1435535E-645.0222669
CCCTAGA1003.0508127E-642.7711522
CAACCGT700.001144597740.7344329
AATGGTG1408.036216E-539.740173145
CTCTGAG1105.87298E-638.8828662
ACTCTTG750.001606724338.0188038
Produced by FastQC (version 0.11.5)