FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132028
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA2670.20222983003605294No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT2530.19162601872330112No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC2430.18405186778562124No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2400.18177962250431726No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC2310.17496288666040538No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT2230.16890356591026146No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC2160.16360166025388553No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT1960.14845335837852577No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT1960.14845335837852577No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1880.14239403762838188No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT1840.1393643772533099No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1660.1257309055654861No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1660.1257309055654861No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC1610.12194383009664617No Hit
GCCATACACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA1570.1189141697215742No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG1550.11739933953403824No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC1550.11739933953403824No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT1540.11664192444027024No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG1510.11436967915896627No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA1450.10982518859635834No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1440.10906777350259036No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT1440.10906777350259036No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG1440.10906777350259036No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG1410.10679552822128639No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA1360.10300845275244645No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1360.10300845275244645No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG1350.10225103765867846No Hit
GTGTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGG1340.10149362256491049No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT1330.1007362074711425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTCC100.0074487836142.584539
GCGAGCC100.0074487836142.584533
ACGTCCG150.007666222141.19293145
CGAATCA752.2813254E-456.477177145
TCACCGG1155.2064024E-755.24941145
CCTTCTG1301.8189894E-1254.8402069
AGAGGCC400.006454235453.46928
TCATATA650.00919687648.874477145
CTTGTAC901.4761335E-647.5281753
GTCATAA1351.573444E-647.064316145
CGCATGA1402.0212847E-645.383446145
TTGTACT952.142102E-645.026694
CCTGGGG657.944145E-443.8721663
CCCTGGG700.001144044240.7384382
TGATGTA1050.001198460140.34084145
GCGCGTA908.222092E-539.606819
AAGCGCG908.222092E-539.606817
AGCGCGT908.222092E-539.606818
GGCGTGA1358.6881846E-539.22026145
ACCTTCT1851.1459633E-1038.5363588
Produced by FastQC (version 0.11.5)