FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50582
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT730.1443201138745008No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAGAGTCATGTCA710.14036613815191176No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC690.13641216242932266No Hit
GTCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT690.13641216242932266No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC690.13641216242932266No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA670.13245818670673362No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT640.12652722312285003No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC610.12059625953896644No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT610.12059625953896644No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG580.11466529595508283No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA560.11071132023249376No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC550.10873433237119925No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT540.1067573445099047No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAGTCTTCTTACTCAT530.10478035664861018No Hit
GCGGTAGGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGA510.1008263809260211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)