FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65883
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC990.1502663813123264No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC920.13964148566398008No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA860.13053443225111183No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC850.12901659001563376No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA830.1259809055446777No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT830.1259809055446777No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT790.11990953660276552No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT760.11535600989633138No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG760.11535600989633138No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA750.11383816766085332No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC700.10624895648346311No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC670.10169542977702897No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT670.10169542977702897No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC660.10017758754155093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)