FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65883
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1100.1669626459025849No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1010.15330206578328248No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC970.1472306968413703No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC830.1259809055446777No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA800.12142737883824356No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT770.11687385213180942No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA770.11687385213180942No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT760.11535600989633138No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG740.11232032542537529No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC720.1092846409544192No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT710.10776679871894117No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT700.10624895648346311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)