FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC130.36424768842813116No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG90.2521714766040908No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT60.1681143177360605No Hit
GGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTA60.1681143177360605No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT60.1681143177360605No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60.1681143177360605No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT60.1681143177360605No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT50.14009526478005044No Hit
TTCTAGGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCG50.14009526478005044No Hit
CTTTAGTAGGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA50.14009526478005044No Hit
CTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTGG50.14009526478005044No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA50.14009526478005044No Hit
GTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAACG50.14009526478005044No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT50.14009526478005044No Hit
GTACTGGAAAGTGCACTTGGACGAACCAGAGTGTAGCTTAACACAAAGCA50.14009526478005044No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA50.14009526478005044No Hit
GGGTGAGCATCAAACTCAAACTACGCCCTGATCGGCGCACTGCGAGCAGT50.14009526478005044No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG40.11207621182404036No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA40.11207621182404036No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC40.11207621182404036No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT40.11207621182404036No Hit
CCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGC40.11207621182404036No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT40.11207621182404036No Hit
GGGTAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGC40.11207621182404036No Hit
GCCTAGGACTCCAGCTCATGCGCCGAATAATAGGTATAGTGTTCCAATGT40.11207621182404036No Hit
CCCTAGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTAC40.11207621182404036No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC40.11207621182404036No Hit
GGTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCG40.11207621182404036No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACA40.11207621182404036No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG40.11207621182404036No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG40.11207621182404036No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT40.11207621182404036No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT40.11207621182404036No Hit
GACCAAGAGCCTTCAAAGCCCTCAGTAAGTTGCAATACTTAATTTCTGTA40.11207621182404036No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT40.11207621182404036No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC40.11207621182404036No Hit
CCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATC40.11207621182404036No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC40.11207621182404036No Hit
TCATAGCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAG40.11207621182404036No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC40.11207621182404036No Hit
GTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAG40.11207621182404036No Hit
GCCCTTACTAGACCAATGGGACTTAAACCCACAAACACTTAGTTAACAGC40.11207621182404036No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT40.11207621182404036No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT40.11207621182404036No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCG40.11207621182404036No Hit
GTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTG40.11207621182404036No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT40.11207621182404036No Hit
GGGATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATT40.11207621182404036No Hit
GTACAATATTCATGGTGGCTGGCAGTAATGTACGAGATACATAGCGGTTG40.11207621182404036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCC100.007812585138.408458
ACCCCTT100.007812585138.408456
TTCCACT100.007812585138.408452
TCCACTA100.007812585138.408453
CTATGTC100.007812585138.408457
CTTCCAC100.007812585138.408451
ACTATGT100.007812585138.408456
Produced by FastQC (version 0.11.5)