FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56513
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC820.14509935766991666No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT760.13448233149894714No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT720.12740431405163413No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC720.12740431405163413No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA710.1256348096898059No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAGAGTCATGTCA700.12386530532797764No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN670.11855679224249288No Hit
GTCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT660.11678728788066463No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC640.11324827915700812No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC610.10793976607152336No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG610.10793976607152336No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT610.10793976607152336No Hit
GCGGTAGGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGA570.10086174862421035No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA570.10086174862421035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGC100.007716439140.839836
CATCTCT204.3192704E-4105.629879
CGCATGA355.5078283E-499.281975145
AACCACG250.001048563984.503897
CAGCGCT300.00216204570.419914
CGGATGC300.00216204570.419919
CGCTAAG300.00216204570.419917
AGCGCTA300.00216204570.419915
GCGCTAA300.00216204570.419916
GTACGGA300.00216204570.419916
ACGAAGA300.00216204570.419917
CCGTGCG350.00398288260.3599249
CACAGCG350.00398288260.3599242
GACGAAG350.00398288260.3599246
CCACGAC350.00398288260.3599249
CGAAGAG350.00398288260.3599248
ACAGCGC400.006756338852.8149343
TGTATAT553.6864667E-451.214483
AGACGAA553.6864667E-451.214485
CTAAGCT700.001209561440.239959
Produced by FastQC (version 0.11.5)