FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9563
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA230.24051030011502667No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT170.17776848269371537No Hit
CTTTAGTAGGTATGCTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA160.16731151312349682No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC150.15685454355327824No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT140.14639757398305972No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG140.14639757398305972No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT130.13594060441284114No Hit
GTTTAGCTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTAAGTGTAAGTTG130.13594060441284114No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG130.13594060441284114No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT130.13594060441284114No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT120.12548363484262262No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAAC120.12548363484262262No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT120.12548363484262262No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC120.12548363484262262No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120.12548363484262262No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC120.12548363484262262No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC120.12548363484262262No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC110.11502666527240407No Hit
GTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATACATAGCGGTTG110.11502666527240407No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACATTCTCCTGA110.11502666527240407No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAGGGGTAGCTTACTG110.11502666527240407No Hit
CTACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGT110.11502666527240407No Hit
GTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAGGT110.11502666527240407No Hit
CTATTAAAGTTTACCACAACCACCACCCCATCATACTCTTTCACCCACAG110.11502666527240407No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG110.11502666527240407No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG110.11502666527240407No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA100.10456969570218551No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA100.10456969570218551No Hit
TTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCA100.10456969570218551No Hit
GTAATAGATAGGGCTCAGGCGTTTGTGTATGATATGTTTGCGGTTTCGAT100.10456969570218551No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG100.10456969570218551No Hit
TCCCTACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCC100.10456969570218551No Hit
CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCA100.10456969570218551No Hit
CTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAGCAAGA100.10456969570218551No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA100.10456969570218551No Hit
GGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTA100.10456969570218551No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG100.10456969570218551No Hit
GTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGG100.10456969570218551No Hit
GTGTAAGTTGGGTGCTTTGTGTTAAGCTACACTCTGGTTCGTCCAAGTGC100.10456969570218551No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT100.10456969570218551No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT100.10456969570218551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)