FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37233
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT560.1504042113179169No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC510.13697526387881717No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC480.12891789541535734No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA460.12354631643971745No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT460.12354631643971745No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT460.12354631643971745No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA450.1208605269518975No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT430.11548894797625761No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG400.10743157951279779No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC390.10474579002497784No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC390.10474579002497784No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC380.1020600005371579No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC380.1020600005371579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)